2016 ANNUAL REVIEW Ministry of Science and Technology

health check-up stations, clinics, and fixed-location disease prevention work. d. Miniaturized low energy consumption nanometer integrated sensors The miniaturized low energy consumption nanometer integrated monitoring sensors developed at NDL can provide terminal applications with a smart sensing function. The sensors rely on electromagnetic induction induced by broadband oscillations in conjunction with integrated passive device back-end process technology to perform real-time monitoring of the vibrations of industrial machinery and check for abnormalities. They can therefore be used to help increase the intelligence of precision machinery and maintain machine reliability. e. 10 nm 2D material transistor process Two-dimensional transition metal chalcogenide compounds are novel substances with extensive potential applications in semiconductor elements. This technology, which involves the use of polysilicon source and drain, employs ion implantation and annealing processes, followed by etching and plasma oxidation to determine whether the gate structure is U-shaped or consists of a tri-gate structure. This approach can be used to incorporate novel structures during the mass production of elements in the future. (6) National Laboratory Animal Center a. Establishment of a mouse neurodegenerative disease behavior analysis platform The causes of degenerative diseases of the central nervous system, such as Parkinson's disease and Alzheimer's disease, have not yet been elucidated, and the development of new drugs to fight these conditions commonly relies on the analysis and assessment of model animals' behavior. The "mouse neurodegenerative disease behavior analysis platform" developed at the National Laboratory Animal Center (NLAC) adopts Bussey-Saksida touch-control screen technology, which is consistent with human medical inspection techniques. The platform's integrated equipment and methods enable researchers to monitor and record mouse behavioral data, which can be used in the preclinical testing of new drugs. b. Finding a new gene causing polycystic kidney disease A polycystic kidney disease is a familial genetic disease for which the causative genes have not yet been discovered. In a global first, researchers at NLAC used novel large-fragment BAC genetic recombinant technology to knock out the gene Wdr19 from the epithelial cells of mouse kidneys, and found that mouse kidneys missing Wdr19 appeared swollen, were full of vesicles, and had severely reduced function, which are all characteristics of polycystic kidney disease. The discovery of the causative role of the gene Wdr19 in polycystic kidney disease by NLAC consequently represents a new opportunity to finds ways to treat this condition. c. Production of ApoE knock-out mice with high application value Hype r l i p i demi a i s mode r n d i sease o f civilization, causes hardening of the blood vessels, induces cardiovascular disease and stroke, and cannot be effectively treated using existing drugs. NLAC used the most advanced CRSIPR/Cas9 gene editing technology to produce Apolipoprotein E (ApoE) gene knock-out mice. These gene knock- out mice have hyperlipidemia, and may have atherosclerotic plaque in the arteries near the heart. These mice are suitable for use in preclinical testing of new drugs for cardiovascular disease induced by hyperlipidemia, and will accelerate new drug R&D in academia and industry. d. Establishment of the NARLabs animals facility international certification assistance team NLAC established the NARLabs animal facility quality improvement team, which has received animal facility quality certification standards and experience from the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC). The assistance team has gained animal facility improvement and new facility establishment certification experience, and members possess AAALAC international certification specialist reviewer qualifications. The team is helping domestic laboratory animal facilities to boost their animal care and experimentation quality, obtain international certification, and thereby establish a basis on which Taiwan's biomedical products can successfully enter international markets. e. Optimized laboratory animal health monitoring technical service NLAC provides a laboratory animal health monitoring technical service intended to ensure animal health and thereby enhance the reliability of experiments. The "IDEXX hematological analysis platform" developed at NLAC needs only 32 μ l blood samples to complete analysis of 20 blood test items, which meets the requirements of continuous analysis of the blood of small laboratory Support for Academic Research Ministry of Science and Technology 65